Coding

Part:BBa_I20289

Designed by: Jerome Bonnet   Group: Endy Lab   (2009-07-11)


PhiRVI excisionase-Freiburg Standard

This protein is a recombination directionality factor (RDF). Association with TP901-1 integrase, and triggers recombination between AttL and attR and excision of the phage genome. Reverse inversion reaction when attL and attR in same DNA molecule. No known catalytic activity per se.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 226
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None